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Does your lab need a reliable centrifuge machine? Digital centrifuge machines separate the components in a sample by replicating and accelerating centrifugation. Carefully designed, they keep you safe while working efficiently. LumBuy offers a variety of blood centrifuge machines, including hand centrifuge machines, refrigerated centrifuge machines, and so on.

When a PRP centrifuge machine spins, it generates a powerful centrifugal force, separating the sample’s contents according to its density. Because it works so well, they are often used in laboratories. For example, in a blood sample that has been centrifuged, the plasma will be at the top, and the red blood cells will be at the bottom. Understanding how centrifuges work will allow you to use this tool better. This guide will show you how it works and provide other relevant information.


BEST CENTRIFUGE MACHINE COLLECTION FOR YOU

  • Centrifuge machine definition
A centrifuge machine, also known as a lab centrifuge machine or plasma centrifuge machine, is a device for separating fluid components by using centrifugal force. It rotates liquids at high speed in a container to separate liquids or solids according to different densities. The denser particles move outward in the radial direction, while the less dense objects move toward the center. In a laboratory centrifuge, the low-density material goes to the top of the tube, while the denser particles settle at the bottom.

  • Principle of centrifuge machine
Centrifugal force causes particles of different densities to separate. The denser ones will sink to the bottom while the lighter ones are suspended.RCF, or relative centrifugal force or G-force, is the acceleration applied to the contents. When the RCF exceeds the frictional and buoyancy forces, the contents will settle away from the axis of rotation. These four factors will also have an effect.

- The density of the sample and solution

- Temperature and viscosity

- The distance the particles are moved

- Velocity

  • History of centrifuge machine
Antonin Prandtl invented the first centrifuge in 1864. People used it in the dairy industry to separate milk and cream. Later, Friedrich Miescher, a Swiss biologist and physician, applied centrifugation in the laboratory for the first time.

  • What is a centrifuge machine used for?
The centrifuge can use centrifugal force to separate various components of liquids, such as plasma, DNA, and urine sediment, according to their density. Centrifuges are commonly used in research and clinical laboratories to purify viruses, proteins, cells, organelles, and nucleic acids.

Industrial centrifuges are used in waste treatment and manufacturing industries to separate immiscible liquids and precipitate solids. Ultracentrifuges and high-speed centrifuges provide extremely high acceleration to separate molecules of different masses and particles down to the nanoscale. Large centrifuges are often used to simulate high acceleration or high gravity environments. Medium-sized centrifuges are used in swimming pools and washing machines. Gas centrifuges are used for isotope separation.

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  • Best Brands
CGOLDENWALL

Cytospin

RegenLab

Despite the complexity of how a centrifuge works, the operation is simple.

The test tube is inserted into one of the ports.

According to the number of samples, insert a test tube filled with water to maintain balance.

Secure the cap and select the settings.

Start the centrifuge.

Wait for the centrifuge to stop spinning

Remove the balance and the sample.

It is important to understand how it works to help you use the centrifuge correctly.

- Balancing the centrifuge

When using the centrifuge, it is essential to balance your samples.

Fill the tubes all evenly with a liquid of similar density.

The tubes are within 0.1 gram of each other.

Place the tubes relative to each other to ensure that gravity is in the center.

If the number of tubes is odd, fill another tube with water for balancing.

  • RPM & RCF
RCF, or relative centrifugal force, is more important than revolutions per minute (RPM). RCF is the force exerted on the contents and is the most important indicator.RCF depends on centrifuge machine RPM and the radius of the rotor.

  • Available rotors
Each model is compatible with different accessories and rotors.

1. Rotors are compatible with containers. Which shapes and sizes of tubes will you use?

2. Centrifuges may have fixed-angle and oscillating rotors. There are also microplate rotors, vertical rotors and PCR strip rotors.

3. Many rotors do not work well at maximum RCF, so you should know their RPM/RCF limits.

  • Temperature range
Many centrifuges do not have cooling or heating options. However, some can offer low temperatures (-10°C or -20°C). It is impossible for all rotors to reach the same temperature when using a refrigerated centrifuge machine (cold centrifuge machine).

  • Safety Features
You have to consider its security features to protect yourself and your surrounding devices. Please note the following four points.

1. The electronic lid lock prevents the lid from being opened accidentally.

2. The imbalance sensor ensures that the centrifuge stops working when it does not get balanced.

3. A sealed rotor prevents the leakage of hazardous substances.

4. Rotor recognition technology ensures that the rotor speed does not exceed the maximum speed.

  • Logistical factors
Centrifuges generate a degree of vibration, so keep it away from sensitive equipment such as laboratory scales.

Some centrifuges can be noisy, especially when running for long periods. The manufacturer will tell you how noisy it is when running it at maximum speed.

  • Centrifugal machine parts
A motor can rotate in the center of the centrifuge. The rotor is connected to the motor. The rotor holds containers of test tubes that can be rotated at an angle of 90 degrees (horizontal centrifuge machine), 45 degrees (fixed angle centrifuge), or no angle (vertical centrifuge machine). The centrifuge tubes can be loaded at the angle at which they rotate. You can also load it into a container that allows for different angles, which is also known as a swinging bucket and is a feature of horizontal centrifuges.

Centrifuges are available with different controls. It will run the motor according to the settings. Some centrifuges have three, two or one processing settings, while some are customizable programs.

The average lab centrifuge machine price on Amazon is 167.66. However, industrial centrifuges may cost a lot.

Benchtop Centrifuge Machine : $1,000 - $5,000

Big/ Large Centrifuge Machine / High Speed Centrifuge Machine: $10,000 - $25,000

Ultra Centrifuge Machine : $10,000 - $50,000

The correct conditions for centrifugation of serum specimens are between 1100-1300 rpm and 15 minutes. Since many blood centrifuges have only one speed, setting it to 15 minutes is fine.

Use warm water, a sponge and a mild detergent.

Avoid products containing chlorine ions or caustic detergents.

Use a plastic scrubbing brush that will not damage the coating.

Use centrifuge lubricant to lubricate rubber seals and barrel tanks.

Perform periodic disinfection.

Check for residue on the rotor.

Perform regular maintenance.

Three parameters can be evaluated when calibrating a centrifuge: time, speed and temperature. You can choose between standard calibration or UKAS calibration. You may require UKAS calibration if compliance with ISO 15189 is required.

If the centrifuge is used frequently, then wear and tear can take a toll on critical components, and we recommend calibration every six months. If you use the centrifuge infrequently, you do not need to calibrate it so often.