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In astronomical research, a UV/Vis spectrophotometer help scientists analyze galaxies, neutron stars, and other celestial objects, providing a wealth of information about the velocities and elements of celestial bodies. In the food industry, a UV/Vis spectrophotometer can further improve the shelf life of foods and ensure safety.

Generally, there are two types to choose from: single beam and double beam spectrophotometer. The two beams have different parts to illuminate and radiate for a double-beam spectrophotometer. The first part illuminates the reference standard, and the second part shines the entire sample. For a single-beam spectrophotometer, on the other hand, the light beam comes from a single light source, which serves to illuminate the specimen and the reference point.

Thanks to its accurate analysis, a UV/Vis spectrophotometer is increasingly popular in forensic analysis, medicine, or pharmacology. Buy the best UV-Vis spectrophotometer to make your job a piece of cake!


Spectrophotometry is a branch of electromagnetic spectroscopy that involves the quantitative measurement of a material's reflectance or transmission properties as a function of wavelength.

Spectrophotometry uses a photometer, called spectrophotometer, that measures the intensity of light beams at different wavelengths. Spectrophotometry is common for ultraviolet, visible, and infrared radiation. Modern spectrophotometers include X-rays, ultraviolet, visible light, infrared, and microwaves.

  • The Best Brands of a spectrophotometer
Pantone UV/Vis spectrophotometer

Agilent UV/Vis spectrophotometer

Konica-Minolta UV/Vis spectrophotometer

Thermo Scientific™ UV/Vis spectrophotometer

FTIR UV/Vis spectrophotometer

NanoDrop UV/Vis spectrophotometer

X-Rite UV/Vis spectrophotometer

Shimadzu UV/Vis spectrophotometer

Datacolor UV/Vis spectrophotometer

D&Y UV/Vis spectrophotometer

CGOLDENWALL UV/Vis spectrophotometer

Vernier International UV/Vis spectrophotometer

AMTAST UV/Vis spectrophotometer

Jenway UV/Vis spectrophotometer

M&A INSTRUMENTS INC UV/Vis spectrophotometer

  • VIS spectrophotometer
A visible spectrophotometer measures absorbance and does quantitative analysis in visible light. You can measure bacterial cell density at 600 nm.

  • UV-VIS spectrophotometer
A UV-Vis spectrophotometer measures the absorbance and quantifies materials under visible or UV light. You can also measure bacterial cell density and the concentration of nucleic acids and proteins.

- Single beam spectrophotometer

It includes a monochromatic light beam (the beam can only pass alternately through the solution), a cuvette, and a photoconverter.

- Features

Simple construction.

Low price.

Mainly used for quantitative analysis.

Measurement results are sometimes affected by power fluctuations, causing significant errors.

Not suitable for the demanding pharmaceutical and quality testing industries.

Require high stability of the light source and detector.

Generally not used for full-band spectral scanning.

Suitable for measuring absorbance or transmittance at specific wavelengths.

- Double beam spectrophotometer

It uses two monochromators to obtain two different wavelengths of monochromatic light. The two beams alternately irradiate the same sample cell at regular intervals.

- Features

Automatic recording.

Fast full waveband scanning.

Eliminate light source instability, detector sensitivity variations, etc.

Suitable for structural analysis.

Measure highly concentrated samples, multi-component mixed samples.

Perform better in turbid samples.

Have higher sensitivity than single beam machines.

- Split beam spectrophotometer

The light from the same monochromator is split into two beams. One reaches the detector directly, and the other passes through the sample to the other detector.

- Features

Allows monitoring of the error of the light source.

It cannot eliminate the effect of the reference.

  • Infrared spectrophotometer
Infrared spectroscopy, in general, refers to infrared spectra greater than 760 nm, which is suitable for organic compounds and can analyze samples of gases, liquids, and solids.

- Features

Fast speed.

Small sample size (a few micrograms to a few milligrams).

Highly characteristic (various substances have their specific infrared spectra).

Analyze various states (gases, liquids, solids) without damaging the sample.

  • Fluorescence spectrophotometer
The fluorescence spectrophotometer is used to scan the fluorescence spectrum.

It is widely used in scientific research, chemical industry, medicine, biochemistry, environmental protection, clinical testing, food testing, teaching experiments, and other fields.

General quantitative analysis.

Infer the conformational changes of molecules in various environments to elucidate the relationship between molecular structure and function.

  • Atomic absorption spectrophotometer
It is mainly suitable for detecting trace components in sample analysis.

It is a powerful tool for materials analysis and trace metal (semi-metallic) elemental analysis.

By watching the video, you will know how to use a spectrophotometer.

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Before purchasing a spectrophotometer, you should consider these factors.

  • Requirements
- Wavelength :

Visible spectrophotometer / UV/visible spectrophotometer / NIR spectrophotometer / NMR devices / atomic absorption spectrophotometer / mercury analyzers / fluorometers / Microspectrophotometers

- Sample throughput:

Spectrophotometers can be classified as single sample and multi-sample units.

- The optical path:

Single-beam, double-beam, and split-beam spectrophotometers are available.

  • Total cost
Detection limits, data quality, analytical operating range, and measurement time are critical in determining purchase selection and cost. Users should also consider whether the unit has customizable or pre-configured detection method buttons, especially when it comes to continuous analysis. The physical footprint and its data output options will also affect the price.

The spectrophotometer measures absorbance (A) and transmittance (T). The intensity of light (I0) measures photons per second. When light passes through a blank sample, it does not absorb light, so it is symbolized as (I). Scientists use blank samples that do not contain chemicals as a reference. They contain everything in the sample cuvette except the material whose absorbance is being measured.

- To calculate transmittance:

Transmittance (T) = It/I­0

It = Light intensity after passing the cuvette (transmitted light)

I0 = Light intensity before passing the cuvette (incident light)

- To calculate absorbance (A):

Absorbance (A) = – log10 T = – log IS/IR

Let's start by analyzing all the parts so that you will know how everything works together.

  • Light source
It ranges from the near-infrared to the ultraviolet range, including the visible light spectrum.

  • Prism
When adjusting the variable wavelength selector, the position of the prism is changed so that different wavelengths are directed toward the sample chamber.

  • Variable wavelength selector
This filters the light so that only a certain wavelength or range of wavelengths is transmitted.

  • Sample chamber
Here you will find transparent tubes, also called cuvettes, containing the samples you want to analyze, called analytes. The wavelength you select with the selector passes through the analyte, which is then detected by a photodetector.

  • Photodetector
The light passing through the analyte sample is directed to the photodetector, made of semiconductor material. Electrons are excited in proportion to the wavelength irradiated to the photodetector. Increasing the intensity of the light produces more electrons, so the signal processor receives a higher current.

  • Display
This component displays the transmittance, absorbance of the sample.

The entrance slit plays a vital role because its size affects the amount of light entering and being measured, thus changing the spectrometer engine's speed and the optical resolution. You can adjust the slit to allow more or less light into the spectrometer.

After the light passes through this entrance slit, it hits the prism and is refracted, then passes through the sample and is measured.

A spectrometer is used to measure changes in physical properties on a spectrum. It collects information about a material based on the amount of infrared, visible, or ultraviolet light it projects.

On the other hand, a spectrophotometer is an instrument that measures light. The exact definition varies from field to field. Generally speaking, "photo" indicates that a spectrophotometer quantitatively measures light intensity in terms of wavelengths. They measure electromagnetic radiation‘s intensity at numerous wavelengths.

  • Function
A colorimeter measures the degree of absorption of a specific color in a sample.

A spectrophotometer measures the transmittance or reflectance of color in a sample as a function of wavelength.

  • Range
Colorimeters only work on a light in the visible part of the electromagnetic spectrum.

A pectrophotometer is suitable for infrared and ultraviolet light as well as visible light.

  • Cost
Compared to a spectrophotometer, a colorimeter is cheaper.

A spectrophotometer has a broader range of functions, including those of a colorimeter. Therefore, it is more expensive than a colorimeter.